Publications
LolA and LolB are conserved in Bacteroidota and are crucial for gliding motility and Type IX secretion
Lipoproteins are key outer membrane (OM) components in Gram-negative bacteria, essential for functions like membrane biogenesis and virulence. Bacteroidota, a diverse and widespread phylum, produce numerous OM lipoproteins that play vital roles in nutrient acquisition, Type IX secretion system (T9SS), and gliding motility. In Escherichia coli, lipoprotein transport to the OM is mediated by the Lol system, where LolA shuttles lipoproteins to LolB, which anchors them in the OM. However, LolB homologs were previously thought to be limited to γ- and β-proteobacteria. This study uncovers the presence of LolB in Bacteroidota and demonstrates that multiple LolA and LolB proteins co-exist in various species. Specifically, in Flavobacterium johnsoniae, LolA1 and LolB1 transport gliding motility and T9SS lipoproteins to the OM. Notably, these proteins are not interchangeable with their E. coli counterparts, indicating functional specialization. Some lipoproteins still localize to the OM in the absence of LolA and LolB, suggesting the existence of alternative transport pathways in Bacteroidota. This points to a more complex lipoprotein transport system in Bacteroidota compared to other Gram-negative bacteria. These findings reveal previously unrecognized lipoprotein transport mechanisms in Bacteroidota and suggest that this phylum has evolved unique strategies to manage the essential task of lipoprotein localization.
Post date: 1 month 2 weeks ago
TamL is a key player of the outer membrane homeostasis in Bacteroidota
In Proteobacteria, the outer membrane protein TamA and the inner membrane-anchored protein TamB form the Translocation and Assembly Module (TAM) complex, which facilitates the transport of autotransporters, virulence factors, and likely lipids across the two membranes. In Bacteroidota, TamA is replaced by TamL, a TamA-like lipoprotein with a lipid modification at its N-terminus that likely anchors it to the outer membrane. This structural difference suggests that TamL may have a distinct function compared to TamA. However, the role of TAM in bacterial phyla other than Proteobacteria remains unexplored.
Our study aimed to elucidate the function of TamL in Flavobacterium johnsoniae, an environmental Bacteroidota. Unlike its homologues in Proteobacteria, we found that TamL and TamB are essential in F. johnsoniae. Through genetic, phenotypic, proteomic, and lipidomic analyses, we show that TamL depletion severely compromises outer membrane integrity, as evidenced by reduced cell viability, altered cell shape, increased susceptibility to membrane-disrupting agents, and elevated levels of outer membrane lipoproteins. Notably, we did not observe an overall decrease in the levels of β-barrel outer membrane proteins, nor substantial alterations in outer membrane lipid composition.
By pull-down assays, we found TamL co-purifying with TamB in F. johnsoniae, suggesting an interaction. Furthermore, we found that while TamL and TamB monocistronic genes are conserved among Bacteroidota, only some species encode multiple TamL, TamB and TamA proteins.
To our knowledge, this study is the first to provide functional insights into a TAM subunit beyond Proteobacteria.
Post date: 1 month 3 weeks ago
Fatal septic shock and Waterhouse-Friderichsen syndrome caused by serovar B Capnocytophaga canimorsus in an immunocompetent patient. Case report and review
Post date: 1 year 1 month ago
A case of septic arthritis caused by Capnocytophaga canimorsus in an HIV patient
Post date: 1 year 1 month ago
Cellulitis and transient bacteremia by Capnocytophaga canis after a cat scratch in an immunocompetent patient
Post date: 1 year 1 month ago
Single cell technologies for monitoring protein secretion heterogeneity
Post date: 1 year 1 month ago
Sepsis caused by Capnocytophaga canimorsus in an immunocompetent patient
Post date: 2 years 12 months ago
Septic shock caused by Capnocytophaga canis after a cat scratch
Post date: 4 years 3 months ago
Capnocytophaga canimorsus Capsular Serovar and Disease Severity, Helsinki Hospital District, Finland, 2000–2017
Post date: 6 years 4 months ago
Capsular serovars of virulent Capnocytophaga canimorsus are shared by the closely related species C. canis and C. cynodegmi
Post date: 6 years 9 months ago
Identification of virulent Capnocytophaga canimorsus isolates by capsular typing
Post date: 7 years 11 months ago
Inactivation of human coagulation factor X by a protease of the pathogen Capnocytophaga canimorsus
Post date: 8 years 3 months ago
Evidence for a LOS and a capsular polysaccharide in Capnocytophaga canimorsus.
Post date: 8 years 3 months ago
Identification of a new lipoprotein export signal in Gram-negative bacteria.
Post date: 8 years 3 months ago
Escape from immune surveillance by Capnocytophaga canimorsus
J Infect Dis. 2007 Feb 1;195(3):375-86. Epub 2006 Dec 19.
Post date: 8 years 5 months ago
Genetic tools for studying Capnocytophaga canimorsus
Appl Environ Microbiol. 2008 Oct;74(20):6369-77. doi: 10.1128/AEM.01218-08. Epub 2008 Aug 22.
Post date: 8 years 5 months ago
Capnocytophaga canimorsus: a human pathogen feeding at the surface of epithelial cells and phagocytes
PloS Pathog. 2008 Sep 26;4(9):e1000164. doi: 10.1371/journal.ppat.1000164.
Post date: 8 years 5 months ago
Capnocytophaga canimorsus resists phagocytosis by macrophages and blocks the ability of macrophages to kill other bacteria
Immunobiology. 2008;213(9-10):805-14. doi: 10.1016/j.imbio.2008.07.019. Epub 2008 Sep 7.
Post date: 8 years 5 months ago
Prevalence of Capnocytophaga canimorsus in dogs and occurrence of potential virulence factors
Microbes Infect. 2009 Apr;11(4):509-14. doi: 10.1016/j.micinf.2009.02.005. Epub 2009 Mar 11.
Post date: 8 years 5 months ago
Resistance of Capnocytophaga canimorsus to killing by human complement and polymorphonuclear leukocytes
Infect Immun. 2009 Jun;77(6):2262-71. doi: 10.1128/IAI.01324-08. Epub 2009 Mar 23
Post date: 8 years 5 months ago
The N-glycan Glycoprotein Deglycosylation Complex (Gpd) from Capnocytophaga canimorsus Deglycosylates Human IgG
PLoS Pathog. 2011 Jun;7(6):e1002118.
Post date: 8 years 5 months ago
The genome and surface proteome of Capnocytophaga canimorsus reveal a key role of glycan foraging systems in host glycoproteins deglycosylation
Mol Microbiol. 2011 Aug;81(4):1050-60. doi: 10.1111/j.1365-2958.2011.07750.x.
Post date: 8 years 5 months ago
Complete genome sequence of the dog commensal and human pathogen Capnocytophaga canimorsus strain 5
J Bacteriol. 2011 Oct;193(19):5558-9. doi: 10.1128/JB.05853-11
Post date: 8 years 5 months ago
Complete genome sequence of the dog commensal and human pathogen Capnocytophaga canimorsus strain 5
J Bacteriol. 2011 Oct;193(19):5558-9. doi: 10.1128/JB.05853-11
Post date: 8 years 5 months ago
Complete genome sequence of the dog commensal and human pathogen Capnocytophaga canimorsus strain 5
J Bacteriol. 2011 Oct;193(19):5558-9. doi: 10.1128/JB.05853-11
Post date: 8 years 5 months ago
The lipopolysaccharide from Capnocytophaga canimorsus reveals an unexpected role of the core-oligosaccharide in MD-2 binding
PLoS Pathog. 2012;8(5):e1002667. doi: 10.1371/journal.ppat.1002667. Epub 2012 May 3.
Post date: 8 years 5 months ago
NMR-based structural analysis of the complete rough-type lipopolysaccharide isolated from Capnocytophaga canimorsus
J Biol Chem. 2014 Aug 22;289(34):23963-76. doi: 10.1074/jbc.M114.571489. Epub 2014 Jul 2.
Post date: 8 years 5 months ago
New iron acquisition system in Bacteroidetes
Infection and Immunity. 83, 1, p. 300-310 11 p.
Post date: 8 years 5 months ago
New iron acquisition system in Bacteroidetes
Infection and Immunity. 83, 1, p. 300-310 11 p.
Post date: 8 years 5 months ago
Draft Genome Sequences of Three Capnocytophaga canimorsus Strains Isolated from Septic Patients
Genome Announc. 2015 May 28;3(3). pii: e00193-15. doi: 10.1128/genomeA.00193-15.
Post date: 8 years 5 months ago
Draft Genome Sequences of Three Capnocytophaga canimorsus Strains Isolated from Healthy Canine Oral Cavities
Genome Announc. 2015 May 28;3(3). pii: e00199-15. doi: 10.1128/genomeA.00199-15.
Post date: 8 years 5 months ago
Draft Genome Sequences of Three Capnocytophaga cynodegmi Strains Isolated from the Oral Cavity of Healthy Dogs
Genome Announc. 2015 May 28;3(3). pii: e00200-15. doi: 10.1128/genomeA.00200-15.
Post date: 8 years 5 months ago
Draft Genome Sequences of Three Capnocytophaga cynodegmi Strains Isolated from the Oral Cavity of Healthy Dogs
Genome Announc. 2015 May 28;3(3). pii: e00200-15. doi: 10.1128/genomeA.00200-15.
Post date: 8 years 5 months ago
Glycan-foraging systems reveal the adaptation of Capnocytophaga canimorsus to the dog mouth
MBio. 2015 Mar 3;6(2):e02507. doi: 10.1128/mBio.02507-14.
Post date: 8 years 5 months ago
Only a subset of C. canimorsus strains is dangerous for humans
Emerg Microbes Infect. 2015 Aug;4(8):e48. doi: 10.1038/emi.2015.48. Epub 2015 Aug 19.
Post date: 8 years 5 months ago
Improvement of identification of Capnocytophaga canimorsus by matrix-assisted laser desorption ionization-time of flight mass spectrometry using enriched database
Diagn Microbiol Infect Dis. 2016 Jan;84(1):12-5. doi: 10.1016/j.diagmicrobio.2015.09.016. Epub 2015 Sep 30.
Post date: 8 years 5 months ago
Modification of the 1-phosphate group during biosynthesis of Capnocytophaga canimorsus lipid A
Infection and Immunity. 2015 Dec 7. pii: IAI.01006-15.
Post date: 8 years 5 months ago
Modification of the 1-phosphate group during biosynthesis of Capnocytophaga canimorsus lipid A
Infection and Immunity. 2015 Dec 7. pii: IAI.01006-15.
Post date: 8 years 5 months ago
