In bacteria, the main model for cell cycle and differentiation is Caulobacter crescentus, a free living alpha-proteobacterium. This bacterium segregates its replicated chromosomes and generates two differentiated cell types at each division. Intense research allowed the characterization of a molecular regulation network controlling cell cycle and differentiation. We discovered that the Brucella abortus cell cycle is surprisingly silmilar to the C. crescentus cell cycle. We now investigate main events of the cell cycle (such as growth and chromosomes replication and segregation) and their regulation. Indeed, our previous data indicate that cell cycle is tightly controlled along a cellular infection. Moreover, cell division events are essential processes, that could become targets for therapy in the future.
In the course of a cellular infection, Brucella abortus is able to control its trafficking. The factors involed in the survival of the bacterium inside host cells are also investigated.
Selected publications over the last 5 years :
Francis N, Poncin K, Fioravanti A, Vassen V, Ong TAP, Rappez L, Letesson JJ, Biondi E, De Bolle X (2017) CtrA controls cell division and outer membrane composition of the pathogen Brucella abortus. Molecular Microbiology 103, 780-797.
De Bolle, X., Crosson, S., Matroule, J.-Y., Letesson, J.J. (2015) Brucella abortus cell cycle and infection are coordinated. Trends Microbiol. 23, 812-821.
Deghelt, M., Mullier, C., Sternon, J.F., Francis, N., Laloux, G., Dotreppe, D., Van der Henst, C., Jacobs-Wagner, C., Letesson, J.J., De Bolle, X. (2014) G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus. Nature Communications 5, 4366.
Van der Henst, C., de Barsy, M., Zorreguieta, A., Letesson, J.J., De Bolle, X. (2013) The Brucella pathogens are polarized bacteria. Microbes and Infection 15, 998-1004.
Van der Henst, C., Beaufays, F., Mignolet, J., Didembourg, C., Colinet, J., Hallet, B., Letesson, J.-J., De Bolle, X. (2012) The histidine kinase PdhS controls cell cycle progression of the pathogenic alphaproteobacterium Brucella abortus. J. Bacteriol. 194, 5305-5314.
Brown, P.J.B., de Pedro, M.A., Kysela, D.T., Van der Henst, C., Kim, J., De Bolle, X., Fuqua, C., Brun, Y. (2012) Polar growth in the Alphaproteobacterial order Rhizobiales. Proc Natl. Acad. Sci. USA 109, 1697-1701.